MES·H2O
Product Description
MES·H2O (MES Monohydrate) is a zwitterionic buffering agent commonly used in biochemical and biological research.
Product:
MES·H2O
CAS:
145224-94-8
Synonym:
MES monohydrate; 2-(N-Morpholino)ethanesulfonic acid hydrate; 4-Morpholineethanesulfonic acid
Structure:
Typical Characteristics
Appearance
White crystalline powder
Density
1.066 g/cm3
Melting point
>300 °C
Molecular Weight
213.25
Odor
Odorless
Purity
≥99.5%
Uses, Applications & Markets
Key applications
Markets
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MES·H2O used in many
industry applications
MES·H2O (MES Monohydrate) is a zwitterionic buffering agent commonly used in biochemical and biological research. Here are some of its applications:
- Biological Buffers: MES·H2O is widely used as a buffering agent in biological and biochemical research, particularly in cell culture media, protein purification, and enzyme assays. It helps maintain a stable pH in various biological systems.
- Electrophoresis: It is used in electrophoresis applications, such as SDS-PAGE and native PAGE, to provide a stable pH environment for the separation of proteins and nucleic acids.
- Tissue Culture: MES·H2O is used in tissue culture media to maintain the pH of the culture environment, promoting optimal cell growth and viability.
- Chromatography: It is used as a buffer in various chromatography techniques, including ion exchange chromatography and size exclusion chromatography, to ensure the stability of proteins and other biomolecules during separation.
- Immunoassays: MES·H2O is used in immunoassays and other diagnostic assays as a buffer to maintain the pH and stability of reagents and samples.
- Protein Crystallization: It is used in protein crystallization studies to provide a stable pH environment, facilitating the formation of high-quality protein crystals for structural analysis.
- Molecular Biology: MES·H2O is used in molecular biology techniques, such as DNA and RNA extraction, to maintain the integrity and stability of nucleic acids during processing.
- Pharmaceuticals: It is used in pharmaceutical formulations as a buffering agent to maintain the stability and efficacy of active pharmaceutical ingredients.
- Analytical Chemistry: MES·H2O is used in analytical chemistry applications as a buffer in various assays and analytical techniques to ensure accurate and reproducible results.
- Environmental Testing: It is used in environmental testing and monitoring applications to buffer samples and maintain the stability of analytes during analysis.