EPPS
Product Description
EPPS (N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)) is a zwitterionic buffering agent commonly used in biological and biochemical research.
Product:
EPPS
CAS:
16052-06-5
Synonym:
4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid; 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid)
Structure:
Typical Characteristics
Appearance
White crystalline powder
Density
1.2684 g/cm3
Melting point
237-239 °C
Molecular Weight
252.33
Purity
≥99.5%
Refractive index
1.6800
Uses, Applications & Markets
Key applications
Markets
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EPPS used in many
industry applications
EPPS (N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)) is a zwitterionic buffering agent commonly used in biological and biochemical research. Here are some of its industrial applications:
- Biological Buffers: EPPS is widely used as a buffering agent in biological and biochemical research, maintaining stable pH conditions in various experiments, particularly in cell culture media and protein purification.
- Electrophoresis: It is utilized in gel electrophoresis and other electrophoretic techniques to ensure consistent pH and ionic strength, which are crucial for accurate separation and analysis of biomolecules.
- Diagnostic Reagents: EPPS is used in the formulation of diagnostic reagents and kits, where it helps maintain optimal conditions for enzymatic reactions and other biochemical assays.
- Pharmaceutical Manufacturing: EPPS is employed in pharmaceutical manufacturing processes to maintain pH stability during drug formulation and production, ensuring the efficacy and stability of the final product.
- Biotechnology: It is used in various biotechnological applications, including DNA sequencing, PCR, and other molecular biology techniques, where precise pH control is essential.
- Research and Development: EPPS is a common reagent in R&D laboratories for developing new biochemical assays, studying enzyme kinetics, and other research activities requiring stable pH conditions.
- Medical Research: In medical research, EPPS is used in studies involving cell cultures, tissue engineering, and other experimental setups where pH maintenance is critical for cell viability and experimental outcomes.
- Environmental Testing: EPPS can be used in environmental testing and monitoring, where maintaining a stable pH is necessary for accurate detection and quantification of pollutants and other environmental parameters.