PIPES
Product Description
PIPES (1,4-Piperazinediethanesulfonic acid) is a commonly used buffering agent in various industrial and research applications due to its ability to maintain stable pH conditions.
Product:
PIPES
CAS:
5625-37-6
Synonym:
1,4-Piperazinediethanesulfonic acid; Piperazine-1,4-bis(2-ethanesulfonic acid); Piperazine-N,N′-bis(2-ethanesulfonic acid)
Structure:
Typical Characteristics
Appearance
White powder
Density
1.4983 g/cm3
Melting point
>300 °C
Molecular Weight
302.37
Purity
≥99%
Refractive index
1.6300
Uses, Applications & Markets
Key applications
Markets
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PIPES used in many
industry applications
PIPES (1,4-Piperazinediethanesulfonic acid) is a commonly used buffering agent in various industrial and research applications due to its ability to maintain stable pH conditions. Here are some of its key uses:
- Biological Research: PIPES is extensively used in biological research laboratories as a buffering agent in cell culture media, enzyme assays, and other biochemical applications. Its stable buffering capacity is essential for maintaining the optimal pH for biological reactions.
- Electrophoresis: It is a popular buffer choice in electrophoresis techniques, particularly in polyacrylamide gel electrophoresis (PAGE). PIPES helps maintain a consistent pH during the separation of proteins and nucleic acids.
- Chromatography: PIPES is used as a buffer in various chromatography techniques, including ion exchange and affinity chromatography. Its ability to maintain a stable pH is crucial for the efficient separation and purification of biomolecules.
- Cell Culture: In cell culture applications, PIPES is used to prepare buffer solutions that help maintain the pH of the culture medium. This is important for the growth and viability of cultured cells.
- Diagnostic Kits: PIPES is used in the formulation of diagnostic kits, where it ensures the stability and reliability of the reagents used in various diagnostic tests, including immunoassays and molecular diagnostics.
- Pharmaceutical Industry: The pharmaceutical industry uses PIPES as a buffering agent in the formulation of various drugs and therapeutic agents. Its ability to maintain a stable pH is essential for the stability and efficacy of pharmaceutical products.
- Enzyme Studies: PIPES is commonly used in enzyme studies and assays to provide a stable pH environment. This is critical for accurately measuring enzyme activity and kinetics.
- Protein Purification: In protein purification processes, PIPES is used as a buffer to maintain the pH of the solution, ensuring the stability and activity of the proteins being purified.
- Environmental Testing: PIPES is employed in environmental testing and monitoring, where it is used to prepare buffer solutions for analyzing water and soil samples. Its stable buffering capacity ensures accurate and reliable results.