DIPSO
Product Description
DIPSO, or 3-(N,N-Bis(2-hydroxyethyl)amino)-2-hydroxypropane sulfonic acid, is a zwitterionic buffer often used in biochemical and molecular biology applications.
Product:
DIPSO
CAS:
68399-80-4
Synonym:
3-(N,N-Bis[2-hydroxyethyl]amino)-2-hydroxypropanesulfonic acid; N,N-Bis(2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid
Structure:
Typical Characteristics
Appearance
White powder
Density
1.494 g/cm3
Melting point
189-192 °C
Molecular Weight
243.28
Purity
≥98%
Refractive index
1.568
Uses, Applications & Markets
Key applications
Markets
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DIPSO used in many
industry applications
DIPSO, or 3-(N,N-Bis(2-hydroxyethyl)amino)-2-hydroxypropane sulfonic acid, is a zwitterionic buffer often used in biochemical and molecular biology applications. Here are some of its key industrial applications:
- Biological Research: DIPSO is widely used in biological research as a buffering agent, maintaining pH stability in various biochemical assays and experiments, including enzyme reactions, cell culture media, and electrophoresis.
- Pharmaceuticals: It is used in the pharmaceutical industry for drug formulation and development, ensuring the stability of active pharmaceutical ingredients (APIs) and improving the efficacy of drug delivery systems.
- Diagnostics: DIPSO is used in diagnostic reagents and kits, providing consistent and reliable pH conditions essential for accurate diagnostic testing, including immunoassays and nucleic acid amplification tests.
- Bioprocessing: It is employed in bioprocessing applications, such as fermentation and cell culture, to control the pH of the growth medium, optimizing the production of biologics like proteins and vaccines.
- Protein Purification: DIPSO is used in protein purification protocols, including chromatography and electrophoresis, to maintain the structural integrity and activity of proteins during separation and analysis.
- Clinical Research: It is utilized in clinical research settings for various analytical and diagnostic procedures, ensuring the accuracy and reliability of experimental results.
- Molecular Biology: DIPSO is used in molecular biology techniques, such as PCR, DNA sequencing, and cloning, providing stable pH conditions necessary for the optimal performance of enzymes and other reagents.
- Biotechnology: It finds applications in biotechnology for the development and production of biotechnological products, including recombinant proteins and monoclonal antibodies, by maintaining optimal pH conditions.
- Environmental Testing: DIPSO is used in environmental testing and monitoring, ensuring accurate measurement of various parameters, such as water quality and pollutant levels, by stabilizing pH conditions.
- Food and Beverage Industry: It is employed in the food and beverage industry for quality control and product development, ensuring the stability and consistency of pH-sensitive ingredients and formulations.