TAE
Product Description
TAE (Tris-Acetate-EDTA) is a buffer solution commonly used in molecular biology and related fields. Its unique properties make it valuable in several specialized applications.
Product:
TAE
CAS:
6850-28-8
Synonym:
Tris acetate; Tris(hydroxymethyl)aminomethane acetate; Trizma acetate
Structure:
Typical Characteristics
Appearance
White crystalline powder
Density
1.09 g/cm3
Melting point
117-118 °C
Molecular Weight
181.19
Odor
Faint vinegar odor
Purity
≥99%
Uses, Applications & Markets
Key applications
Markets
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TAE used in many
industry applications
TAE (Tris-Acetate-EDTA) is a buffer solution commonly used in molecular biology and related fields. Its unique properties make it valuable in several specialized applications. Here are some of its key industrial and research uses:
- Electrophoresis: TAE buffer is widely used in agarose gel electrophoresis for the separation of nucleic acids (DNA and RNA). It helps maintain the pH and ionic strength necessary for the accurate separation and analysis of nucleic acids.
- Molecular Biology: In molecular biology, TAE buffer is used in various techniques, including restriction digestion and DNA amplification. It helps stabilize nucleic acids and enzymes during these processes.
- DNA Sequencing: TAE buffer is employed in DNA sequencing protocols to ensure optimal conditions for the separation of DNA fragments and accurate sequencing results.
- Protein Analysis: While less common than in nucleic acid work, TAE buffer can be used in protein electrophoresis for the separation and analysis of proteins under specific conditions.
- Research and Development: TAE buffer is used in research and development for studying nucleic acid properties and optimizing molecular biology techniques. Researchers utilize it to explore new applications and improve existing protocols.
- Quality Control: In laboratories, TAE buffer is used for quality control of various molecular biology products and reagents. It ensures that the conditions for nucleic acid analysis are consistent and reliable.