Product Description

N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid, commonly known as TES, is a chemical compound used in various laboratory and biotechnological applications.

Product:

TES

CAS:

7365-44-8

Synonym:

N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid; 2-[(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]ethanesulfonic acid

Structure:

Typical Characteristics

Appearance

White powder

Density

1.260 g/cm3

Melting point

216-218°C

Molecular Weight

229.25

Odor

Odorless

Purity

≥99%

Refractive index

1.550

Uses, Applications & Markets

Key applications

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We Offer TES
in various grades

A few of the grades available are listed below:

TES used in many
industry applications

N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid, commonly known as TES, is a chemical compound used in various laboratory and biotechnological applications. Here's a list of some of its uses:

  1. Buffer Solution: TES is commonly used as a buffering agent in biological and biochemical experiments. It helps maintain a stable pH in solutions, particularly in the physiological pH range, making it suitable for cell culture media and enzyme assays.
  2. Electrophoresis: TES buffer is used in gel electrophoresis techniques for the separation and analysis of nucleic acids and proteins. It provides the appropriate pH conditions for efficient electrophoretic mobility of biomolecules.
  3. Enzyme Studies: TES buffer is employed in enzymology studies to maintain optimal pH conditions for enzyme activity. It can be used in kinetic assays, enzyme kinetics, and enzyme purification protocols.
  4. Cell Culture: TES buffer is used in cell culture media to maintain the pH of the growth environment for various cell types. It provides a stable pH environment that mimics physiological conditions, promoting cell viability and growth.
  5. PCR (Polymerase Chain Reaction): TES buffer is sometimes used in PCR reactions as a component of the reaction mix. It helps maintain the pH stability during the thermal cycling process, ensuring efficient amplification of DNA targets.
  6. Protein Crystallization: TES buffer is used in protein crystallization experiments as a crystallization agent or as a component of crystallization buffers. It provides suitable pH conditions and ionic strength for protein crystallization and crystal growth.
  7. Biophysical Studies: TES buffer is employed in various biophysical studies, such as spectroscopy, calorimetry, and structural biology. It provides a stable pH environment for investigating the structure, stability, and interactions of biomolecules.
  8. Chemical Synthesis: TES can also be used as a reagent or solvent in organic synthesis reactions. Its mild properties and water solubility make it suitable for certain chemical transformations and as a reaction medium.